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portada in situ detection of dna damage: methods and protocols
Type
Physical Book
Illustrated by
Publisher
Author
Year
2010
Language
English
Pages
313
Format
Paperback
Dimensions
22.9x15.2x1.8 cm
Weight
0.45 kg.
ISBN
1617372722
ISBN13
9781617372728
Categories

in situ detection of dna damage: methods and protocols

Vladimir V. Didenko (Illustrated by) · Humana · Paperback

in situ detection of dna damage: methods and protocols - Didenko, Vladimir V.

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Synopsis "in situ detection of dna damage: methods and protocols"

Detection and analysis of DNA damage is of critical importance in a variety of biological disciplines studying apoptosis, cell cycle and cell di- sion, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and radiobiological research, and others. Individual cells within the same tissue or in cell culture may vary in the extent of their DNA damage and, consequently, can display different re- tions to it. These differences between individual cells in the same cell popu- tion are detected using in situ approaches. In situ is a Latin term meaning "on site" or "in place." It is used to denote the processes occurring or detected in their place of origin. In mole- lar and cell biology this usually refers to undisrupted mounted cells or tissue sections. In that meaning "in situ" is used as part of the terms "in situ PCR," "in situ transcription," "in situ hybridization," "in situ end labeling," and "in situ ligation." Sometimes the "in situ" term is applied at the subcellular level to cells disrupted in the process of analysis, for example, in the detection of specific sequences in chromosomes using fluorescent in situ hybridization (FISH). Historically, the term was used primarily in methods dealing with nucleic acids.

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