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portada Protein Phosphorylation Analysis by Electrospray Mass Spectrometry: A Guide to Concepts and Practice
Type
Physical Book
Publisher
Author
Year
2010
Language
English
Pages
396
Format
Hardcover
ISBN
0854041850
ISBN13
9780854041855
Edition No.
1
Categories

Protein Phosphorylation Analysis by Electrospray Mass Spectrometry: A Guide to Concepts and Practice

Wolf D Lehmann (Author) · Royal Society Of Chemistry · Hardcover

Protein Phosphorylation Analysis by Electrospray Mass Spectrometry: A Guide to Concepts and Practice - Wolf D Lehmann

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Synopsis "Protein Phosphorylation Analysis by Electrospray Mass Spectrometry: A Guide to Concepts and Practice "

Protein phosphorylation analysis is a central theme in current analytical biochemistry, cell biology and systems biology. Due to its versatility, specificity and sensitivity, mass spectrometry has developed into a key technology in this field. A set of minor and major instrumental innovations mean that mass spectrometers now exhibit a level of performance, a stability of operation, a relative ease of use, and productivity, which would once have been hard to imagine. This book guides the reader through this prolific field by presenting a collection of personal views and selected examples which cover all the important principles with a focus on electrospray ionization mass spectrometry. It covers: phosphorylation analysis at the peptide, protein and proteome level; manual and automated data evaluation; phosphopeptide enrichment; quantitative aspects; element mass spectrometry; individual analytical strategies, and hints to useful internet resources. This book provides students, graduate students, post-Docs and senior scientists from related areas with a better understanding on molecular protein phosphorylation analysis. Its highest aim is to strengthen the reader's ability to develop a personal, well-founded opinion on original manuscripts published in this field.

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