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portada Sh2 Domains: Functional Modules and Evolving Tools in Biology (Methods in Molecular Biology, 2705)
Type
Physical Book
Publisher
Author
Language
English
Pages
393
Format
Hardcover
ISBN13
9781071633922
Edition No.
1

Sh2 Domains: Functional Modules and Evolving Tools in Biology (Methods in Molecular Biology, 2705)

Carlomagno Teresa,Köhn Maja (Author) · Humana · Hardcover

Sh2 Domains: Functional Modules and Evolving Tools in Biology (Methods in Molecular Biology, 2705) - Carlomagno Teresa,Köhn Maja

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Synopsis "Sh2 Domains: Functional Modules and Evolving Tools in Biology (Methods in Molecular Biology, 2705)"

This volume looks at the latest methods used to study and modulate the biological function and mechanisms of SH2 domains. The chapters in this book are organized into five parts. Part One presents methodology aimed at determining the structures and dynamics of SH2 domains and their complexes with phosphopeptides. Part Two discusses techniques to understand and predict interactions of SH2 domains by measuring or calculating their affinity to phosphopeptides. Part Three focuses on inhibitors of SH2 domains that lead the way for chemical tool development and drug discovery. Part Four describes how to evolve and engineer SH2 domains with specific binding properties, and Part Five explores how to measure the regulation of protein tyrosine phosphatase activity through allosteric binding of peptides to SH2 domains and condensation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, SH2 Domains: Functional Modules and Evolving Tools in Biology is a valuable resource for researchers, working in the biophysical and biochemical field, who want to learn more about this exciting and versatile class of regulatory and signaling domains.

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